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Here are the 24 US AI startups that have raised $100M or more in 2025

  • In 2024, 49 AI startups in the U.S. raised $100 million or more, with three raising more than one mega-round and seven exceeding $1 billion.
  • In 2025, the AI industry's momentum in the U.S. continues, with multiple billion-dollar rounds and more AI mega-rounds in Q1 compared to 2024.
  • Notable U.S. AI startups raising funds in 2025 include Glean, Anysphere, Snorkel AI, LMArena, TensorWave, SandboxAQ, Runway, OpenAI, Nexthop AI, Insilico Medicine, and Celestial AI.
  • Other notable fundraisers in 2025 include Lila Sciences, Reflection.Ai, Turing, Shield AI, Anthropic, Together AI, Lambda, Abridge, Eudia, EnCharge AI, Harvey, ElevenLabs, and Hippocratic AI.
  • Various rounds led by prominent investors such as Thrive Capital, Lightspeed Venture Partners, General Atlantic, SoftBank, and Sequoia, among many others, fueled the AI ecosystem growth in the U.S.
  • Several companies surpassed valuation milestones, with Glean valued at $7.25 billion, Anysphere at nearly $10 billion, OpenAI at $300 billion, and Anthropic at $61.5 billion.
  • The U.S. landscape for AI funding is diverse and includes sectors like enterprise search, AI coding tools, data labeling, AI models, AI infrastructure, autonomous systems, and more.
  • AI startups in 2025 are attracting significant capital, highlighting the continued interest and growth opportunities in the AI space across various verticals.
  • The AI investment trend is expected to reflect a positive trajectory for the remainder of the year as the industry witnesses robust funding activities and valuation expansions.
  • The wide array of fundraising rounds and increasing valuations signify the eagerness of investors to back innovative AI solutions and technologies in the U.S. market.

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