A naukri.com initiative
Bioengineer
2d
99
Image Credit: Bioengineer
Efficient Mitochondrial A-to-G Base Editors Developed
- Scientists have developed a new generation of mitochondrial DNA base editors that are significantly more efficient and precise compared to previous versions.
- These enhanced TadA-8e-based adenine base editors demonstrate increased editing activity and an expanded range of editable sequence contexts within mitochondria.
- Mitochondria, crucial for energy production, have been challenging to manipulate due to low editing efficiency and limited targeting capabilities.
- The engineered eTd-mtABEs exhibit editing efficiencies of up to 87% in human cellular models, marking a significant advancement in mitochondrial genome engineering.
- These editors show exceptional specificity, minimizing off-target effects at both DNA and RNA levels, crucial for safe and precise therapeutic applications.
- The eTd-mtABEs utilize DNA nickases for strand-selective editing, enhancing efficiency and reducing the risk of deleterious double-stranded DNA breaks.
- In vivo studies in rat models demonstrate editing efficiencies up to 145 times higher than previous editing tools, making them a premier platform for mitochondrial genome manipulation.
- Researchers successfully generated heritable mitochondrial disease models in rats using these editors, showcasing their potential in exploring disease mechanisms and therapeutics.
- The innovation in eTd-mtABEs strikes a balance between editing efficiency and specificity, crucial for future therapeutic advancements in mitochondrial gene editing.
- This breakthrough opens avenues for precise correction of pathogenic mitochondrial variants, potentially revolutionizing the treatment of mitochondrial diseases.
Read Full Article
5 Likes
For uninterrupted reading, download the app