A naukri.com initiative
Bioengineer
2w
232
Image Credit: Bioengineer
Shaking Disrupts Stem Cell Clocks via TEAD Pathway
- Research reveals how mechanical stimulation alters circadian rhythms in induced pluripotent stem cells (iPSCs) during differentiation into osteogenic lineages, shedding light on stem cell biology and tissue engineering applications.
- The study focuses on the impact of shaking culture on the circadian clock machinery of iPSCs undergoing osteogenic differentiation, with a particular emphasis on the TEAD-Fbxl3-CRY axis as a key mediator of circadian regulation in response to mechanical cues.
- Shaking culture leads to a significant reduction in circadian amplitudes and dampened oscillations in core clock genes, disrupting the temporal signaling required for precise gene expression during differentiation.
- The TEAD-Fbxl3-CRY axis integrates mechanical forces from shaking culture with circadian rhythms, offering a molecular framework for manipulating circadian dynamics in stem cells under mechanical stress.
- Understanding the interplay between circadian biology and stem cell fate decisions is crucial for enhancing the predictability and efficacy of stem cell-derived therapies, especially in osteogenic applications for bone repair and regeneration.
- This study underscores the importance of mechanotransduction in stem cell biology, revealing how mechanical stimuli influence not only gene expression and cytoskeletal organization but also circadian timekeeping mechanisms.
- By elucidating the TEAD-Fbxl3-CRY pathway's role in circadian attenuation under mechanical stimulation, the research offers insights into designing biomimetic culture systems that optimize differentiation protocols with temporal precision.
- The findings highlight the complexity of in vitro differentiation systems and prompt investigations into how mechanical forces in vivo affect circadian biology during bone development and remodeling.
- This work sets the stage for future studies exploring the broader impact of mechanical environments on circadian clocks in various stem cell types and tissues, offering potential implications for aging, disease susceptibility, and tissue homeostasis.
- The discovery of the TEAD-Fbxl3-CRY axis as a link between mechanical cues and circadian modulation in iPSC-derived osteogenic cells reshapes our understanding of temporal regulation in stem cell fate determination and underscores the relevance of chronobiology in regenerative medicine.
Read Full Article
14 Likes
For uninterrupted reading, download the app